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rage shrna vectors  (OriGene)


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    Structured Review

    OriGene rage shrna vectors
    Rage Shrna Vectors, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rage+shrna+vectors/Ager+Mouse+shRNA+Plasmid/pm36394567-48-22-33
    Average 91 stars, based on 1 article reviews
    rage shrna vectors - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Knockdown:

    Article Title: RAGE ablation attenuates glioma progression and enhances tumor immune responses by suppressing galectin-3 expression.
    Article Snippet: Background.. Malignant gliomas consist of heterogeneous cellular components that have adopted multiple overlapping escape mechanisms that overcome both targeted and immune-based therapies.. The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily that is activated by diverse proinflammatory ligands present in the tumor microenvironment.

    Generated:

    Article Title: RAGE ablation attenuates glioma progression and enhances tumor immune responses by suppressing galectin-3 expression.
    Article Snippet: Background.. Malignant gliomas consist of heterogeneous cellular components that have adopted multiple overlapping escape mechanisms that overcome both targeted and immune-based therapies.. The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily that is activated by diverse proinflammatory ligands present in the tumor microenvironment.

    Stable Transfection:

    Article Title: RAGE ablation attenuates glioma progression and enhances tumor immune responses by suppressing galectin-3 expression.
    Article Snippet: Background.. Malignant gliomas consist of heterogeneous cellular components that have adopted multiple overlapping escape mechanisms that overcome both targeted and immune-based therapies.. The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily that is activated by diverse proinflammatory ligands present in the tumor microenvironment.

    Expressing:

    Article Title: RAGE ablation attenuates glioma progression and enhances tumor immune responses by suppressing galectin-3 expression.
    Article Snippet: Background.. Malignant gliomas consist of heterogeneous cellular components that have adopted multiple overlapping escape mechanisms that overcome both targeted and immune-based therapies.. The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily that is activated by diverse proinflammatory ligands present in the tumor microenvironment.

    shRNA:

    Article Title: RAGE ablation attenuates glioma progression and enhances tumor immune responses by suppressing galectin-3 expression.
    Article Snippet: Background.. Malignant gliomas consist of heterogeneous cellular components that have adopted multiple overlapping escape mechanisms that overcome both targeted and immune-based therapies.. The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily that is activated by diverse proinflammatory ligands present in the tumor microenvironment.

    Plasmid Preparation:

    Article Title: RAGE ablation attenuates glioma progression and enhances tumor immune responses by suppressing galectin-3 expression.
    Article Snippet: Background.. Malignant gliomas consist of heterogeneous cellular components that have adopted multiple overlapping escape mechanisms that overcome both targeted and immune-based therapies.. The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily that is activated by diverse proinflammatory ligands present in the tumor microenvironment.



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    (A) mRNA expression profile of RAGE in different gastric cancer cells. (B) Protein expression and quantification of RAGE in gastric cancer cells. (C) RAGE was stably knocked down in <t>SGC7901</t> cells. (D) The peritoneal nodules in nude mice administered with glucose-derived AGEs for two months. (E) The quantification of peritoneal nodules. ( ** P <0.01). (F) The inhibitory effect of RAGE knock-down on the AGEs-induced Sp1 and MMP2 expression in vivo .
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    Image Search Results


    (A) mRNA expression profile of RAGE in different gastric cancer cells. (B) Protein expression and quantification of RAGE in gastric cancer cells. (C) RAGE was stably knocked down in SGC7901 cells. (D) The peritoneal nodules in nude mice administered with glucose-derived AGEs for two months. (E) The quantification of peritoneal nodules. ( ** P <0.01). (F) The inhibitory effect of RAGE knock-down on the AGEs-induced Sp1 and MMP2 expression in vivo .

    Journal: Oncotarget

    Article Title: Glucose-derived AGEs enhance human gastric cancer metastasis through RAGE/ERK/Sp1/MMP2 cascade

    doi: 10.18632/oncotarget.22185

    Figure Lengend Snippet: (A) mRNA expression profile of RAGE in different gastric cancer cells. (B) Protein expression and quantification of RAGE in gastric cancer cells. (C) RAGE was stably knocked down in SGC7901 cells. (D) The peritoneal nodules in nude mice administered with glucose-derived AGEs for two months. (E) The quantification of peritoneal nodules. ( ** P <0.01). (F) The inhibitory effect of RAGE knock-down on the AGEs-induced Sp1 and MMP2 expression in vivo .

    Article Snippet: In order to stably knock-down RAGE expression of SGC7901 cells, lentiviral expression vectors containing RAGE-specific shRNAs and green fluorescent protein (GFP) were constructed by GeneChem (Shanghai, China).

    Techniques: Expressing, Stable Transfection, Derivative Assay, Knockdown, In Vivo

    (A) Wound healing assays with SGC7901 treated with 100μg/ml AGEs or unmodified BSA was recorded at 0 and 48 hours. (B) The mean distances between wound edges of SGC7901 cells at 0 and 48 hours. (C, D) Transwell migration and invasion assays for SGC7901 cells treated with 100μg/ml AGEs or unmodified BSA for 2 days. (E, F) The number of migration and invasion cells. All experiments were carried out in triplicate. ( ** p <0.05).

    Journal: Oncotarget

    Article Title: Glucose-derived AGEs enhance human gastric cancer metastasis through RAGE/ERK/Sp1/MMP2 cascade

    doi: 10.18632/oncotarget.22185

    Figure Lengend Snippet: (A) Wound healing assays with SGC7901 treated with 100μg/ml AGEs or unmodified BSA was recorded at 0 and 48 hours. (B) The mean distances between wound edges of SGC7901 cells at 0 and 48 hours. (C, D) Transwell migration and invasion assays for SGC7901 cells treated with 100μg/ml AGEs or unmodified BSA for 2 days. (E, F) The number of migration and invasion cells. All experiments were carried out in triplicate. ( ** p <0.05).

    Article Snippet: In order to stably knock-down RAGE expression of SGC7901 cells, lentiviral expression vectors containing RAGE-specific shRNAs and green fluorescent protein (GFP) were constructed by GeneChem (Shanghai, China).

    Techniques: Migration

    (A) Representative images of immunohistochemistry showed overexpression of Sp1 in human gastric cancer tissues (200×). (B) Suppression of Sp1 protein expression in SGC7901 cells with Sp1sepcific siRNAs. (C, D) The inhibitory effects of Sp1 knockdown on AGEs-induced cells migration and invasion. (E, F) Reduction of AGEs-induced MMP2 protein expression and enzymatic activity in SGC7901 cells by down-regulating Sp1 expression. All operations were repeated three times. ( ** p <0.05).

    Journal: Oncotarget

    Article Title: Glucose-derived AGEs enhance human gastric cancer metastasis through RAGE/ERK/Sp1/MMP2 cascade

    doi: 10.18632/oncotarget.22185

    Figure Lengend Snippet: (A) Representative images of immunohistochemistry showed overexpression of Sp1 in human gastric cancer tissues (200×). (B) Suppression of Sp1 protein expression in SGC7901 cells with Sp1sepcific siRNAs. (C, D) The inhibitory effects of Sp1 knockdown on AGEs-induced cells migration and invasion. (E, F) Reduction of AGEs-induced MMP2 protein expression and enzymatic activity in SGC7901 cells by down-regulating Sp1 expression. All operations were repeated three times. ( ** p <0.05).

    Article Snippet: In order to stably knock-down RAGE expression of SGC7901 cells, lentiviral expression vectors containing RAGE-specific shRNAs and green fluorescent protein (GFP) were constructed by GeneChem (Shanghai, China).

    Techniques: Immunohistochemistry, Over Expression, Expressing, Knockdown, Migration, Activity Assay